![]() ![]() Suggesting that vitamin C may also have important cellularįunctions. Indicated that vitamin C is the most important water-solubleĪntioxidant in human plasma and mammalian cells ( 10, 11), Macrophages can survive in the host cell for a substantial period tb can induce necrosis ( 6), apoptosis ( 7) and autophagy in macrophages ( 8). tb colonization and reproduction ( 5), but they are also the first line ofĭefense against M. Individual differences associated with peripheral blood mononuclearĬells, which is conducive to the reproduction of experimentalĬells are an ideal tool for studying immunity and inflammation.Īlveolar macrophages are not only the primary siteįor M. Stable genetic background, and do not possess the problem of THP-1 cellsĪre easy to cultivate and expand in the laboratory, have a more Macrophage-related mechanisms and signaling pathways. THP-1 cells are widely used in the study of monocyte and Theoretical and experimental basis for treating tuberculosis is Which poses a serious threat to public health ( 3). tb ( 2), resulting in ~1.7 million mortalities, ~10 million individuals become infected with M. It can invade all organs of the bodyĪnd is the most common cause of tuberculosis. The pathogen that causes tuberculosis ( 1). ![]() Overall, these results suggested that vitamin C may reduce lung damage caused by M. tb infection. The results demonstrated that vitamin C reduced the expression of M. tb‑induced apoptosis‑related proteins (Cleaved‑caspase‑9, Cleaved‑caspase‑3, Bcl‑2, Cyt‑c) and inflammatory factors (IL‑1β, IL‑6, NLRP3, TNF‑α, IL‑8, NF‑κB) in THP‑1 cells and reduced apoptosis. The results demonstrated that vitamin C markedly attenuated cellular damage caused by M. tb infection. The effect of vitamin C on M. tb infection was also assessed using Balb/c mice pulmonary injury was assessed by H&E staining of the lung tissue. In the present study, MTT assay, reverse transcription‑quantitative PCR, EdU cell proliferation assay, western blotting, immunohistochemistry, flow cytometry and TUNEL staining assays were used to assess the cell viability, inflammation and apoptotic levels of THP‑1 cells induced by M. tb following vitamin C treatment. The present study aimed to further investigate the effects of vitamin C on M. tb infection‑induced THP‑1 cell injury and its mechanism. However, whether vitamin C can protect THP‑1 cells from M. tb infection has not yet been reported. Its biological efficacy depends on its redox abilities and its role as a cofactor in several enzymatic reactions. Vitamin C is a trace element for the human body. Macrophages are important for the progression of tuberculosis, as they affect the release of various inflammatory cytokines, including IL‑1β, IL‑6 and TNF‑α. The THP‑1 cell line is a cell model for studying the function, mechanism and signaling pathways of macrophages macrophages are the primary host cells of M. tb. Tuberculosis (TB) is a chronic and fatal zoonotic infectious disease caused by Mycobacterium tuberculosis ( M. tb) infection. ![]()
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